3ATP
Structure of the ligand binding domain of the bacterial serine chemoreceptor Tsr with ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 35 |
| Detector technology | CCD |
| Collection date | 2004-06-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 51.972, 133.737, 39.571 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.570 - 2.500 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.071 | 0.331 |
| Number of reflections | 9819 | |
| <I/σ(I)> | 16.7 | 4.5 |
| Completeness [%] | 98.4 | 100 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 12-14% PEG 1000, 0.1M Acetate buffer, 80-100mM ammonium acetate , pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
