3AT6
Side-necked turtle (Pleurodira, Chelonia, REPTILIA) hemoglobin: cDNA-derived primary structures and X-ray crystal structures of Hb A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-24 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 65.994, 65.994, 284.823 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.150 - 2.350 |
| Rwork | 0.240 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3at5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.200 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | EPMR |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.150 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.136 | 0.354 |
| Number of reflections | 14223 | |
| <I/σ(I)> | 5.7 | 3.5 |
| Completeness [%] | 87.2 | 99.7 |
| Redundancy | 3.28 | 4.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
