3ASZ

CMP-complex structure of uridine kinase from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-22
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	68.730, 126.942, 61.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.250
R-factor	0.1962
Rwork	0.196
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	uridine cytidine kinase2 from Homo Sapiens
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge		0.858
Number of reflections	29582
<I/σ(I)>		7.8
Redundancy		5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	50%(w/v) PEG 200, 0.1M Tris-HCl, pH 8.0, VAPOR DIFFUSION, temperature 293K