3ASS
Crystal structure of P domain from Norovirus Funabashi258 stain in the complex with Lewis-b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-11-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 74.690, 74.690, 106.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.230 - 1.600 |
| R-factor | 0.18565 |
| Rwork | 0.185 |
| R-free | 0.20171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3asr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.075 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
| Rmerge | 0.043 | 0.032 | 0.238 |
| Number of reflections | 88432 | ||
| <I/σ(I)> | 25.5 | ||
| Completeness [%] | 99.9 | 97.6 | 100 |
| Redundancy | 11.2 | 10.8 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 4.6 | 298 | PEG 6000, hexanediol, pH 4.6, hanging drop, temperature 298K |
