3ASQ

Crystal structure of P domain from Norovirus Funabashi258 stain in the complex with H-antigen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2008-11-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 31
Unit cell lengths	74.690, 74.690, 107.106
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.260 - 1.600
R-factor	0.17915
Rwork	0.178
R-free	0.19353
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.050
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.080	0.059	0.389
Number of reflections	88096
<I/σ(I)>	12.9
Completeness [%]	100.0	99.7	100
Redundancy	11.3	10.7	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	4.6	298	PEG 6000, hexanediol, pH 4.6, hanging drop, temperature 298K