3ASN
Bovine heart cytochrome C oxidase in the fully oxidized state measured at 1.7470 angstrom wavelength
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 50 |
| Detector technology | CCD |
| Collection date | 2009-06-14 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.7470 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 181.826, 204.098, 177.829 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| Rwork | 0.150 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dyr |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.090 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 136.000 | 3.030 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 132667 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 6.8 | 277 | 40mM sodium phosphate, 0.2% decylmaltoside , pH 6.8, BATCH, temperature 277K |
