3ASI
Alpha-Neurexin-1 ectodomain fragment; LNS5-EGF3-LNS6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 72.910, 79.380, 78.135 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.070 - 2.300 |
| R-factor | 0.1997 |
| Rwork | 0.197 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c4r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.124 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.070 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.083 | 0.271 |
| Number of reflections | 20732 | |
| <I/σ(I)> | 20.9 | 6.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 18% PEG3000, 0.1M sodium acetate and 0.3M sodium malonate pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293KK |
