3ASH
MamA D159K mutant 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.143, 77.477, 101.811 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.670 - 2.410 |
| R-factor | 0.1851 |
| Rwork | 0.183 |
| R-free | 0.21530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3as5 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.832 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.083 | 0.050 | 0.468 |
| Number of reflections | 22006 | ||
| <I/σ(I)> | 12.1 | ||
| Completeness [%] | 94.8 | 96.4 | 100 |
| Redundancy | 8.1 | 7.1 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 286 | Ammonium sulfate, Glycerol, Tris, NaCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
