3ASA
Crystal structure of apo-LL-diaminopimelate aminotransferase from Chlamydia trachomatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-05 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.978483 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 110.246, 110.246, 205.626 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.050 |
| R-factor | 0.22899 |
| Rwork | 0.227 |
| R-free | 0.27683 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.433 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0055) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.130 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 40054 | |
| <I/σ(I)> | 2 | |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 10% PEG6000, 2.0M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
