3AS4
MamA AMB-1 C2221
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.776, 78.282, 95.999 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.110 - 2.330 |
| R-factor | 0.218 |
| Rwork | 0.215 |
| R-free | 0.27040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3as5 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.624 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 31.970 | 31.970 | 2.390 |
| High resolution limit [Å] | 2.330 | 6.360 | 2.350 |
| Rmerge | 0.087 | 0.054 | 0.467 |
| Number of reflections | 12885 | ||
| <I/σ(I)> | 13.9 | ||
| Completeness [%] | 96.9 | 97.8 | 100 |
| Redundancy | 10.4 | 8.9 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 286 | Ammonium sulfate, Glycerol, Tris, NaCl, pH 8.9, VAPOR DIFFUSION, SITTING DROP, temperature 286K |
