3AQY
Crystal structure of Plodia interpunctella beta-GRP/GNBP3 N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.350, 38.034, 55.630 |
| Unit cell angles | 90.00, 117.34, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.580 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aqx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.466 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.046 | 0.130 |
| Number of reflections | 27224 | |
| <I/σ(I)> | 25.6 | 9.5 |
| Completeness [%] | 97.5 | 85.3 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M ammonium sulfate, 0.1M MES, 30% w/v PEG 5000 MME, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
