3AQX
Crystal structure of Bombyx mori beta-GRP/GNBP3 N-terminal domain with laminarihexaoses
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 |
| Unit cell lengths | 74.210, 74.210, 47.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.050 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ie4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.512 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.172 | 0.461 |
| Number of reflections | 16156 | |
| <I/σ(I)> | 9.1 | 2 |
| Completeness [%] | 98.3 | 87.9 |
| Redundancy | 6.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M ammonium sulfate, 0.1M HEPES sodium buffer, 10% (w/v) PEG 4000, 5mM laminarihexaose, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
