3AQU
Crystal structure of a class V chitinase from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-03-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.574, 75.079, 97.347 |
| Unit cell angles | 73.63, 73.34, 68.47 |
Refinement procedure
| Resolution | 50.000 - 2.010 |
| R-factor | 0.1978 |
| Rwork | 0.196 |
| R-free | 0.22715 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3alf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.155 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.336 | |
| Number of reflections | 116512 | |
| <I/σ(I)> | 7.6 | 1.9 |
| Completeness [%] | 94.5 | 88.7 |
| Redundancy | 2.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 100mM trisodium citrate, 20% PEG10000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
