3AQK
Structure of bacterial protein (apo form I)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-06 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9790, 0.97928, 0.98317 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 130.848, 130.848, 149.639 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.970 - 3.650 |
| R-factor | 0.279 |
| Rwork | 0.279 |
| R-free | 0.29500 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.780 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Number of reflections | 15761 | |
| <I/σ(I)> | 14.8 | 2 |
| Completeness [%] | 98.5 | 92.6 |
| Redundancy | 4.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 100mM Tris-Cl, 0.5M sodium chloride, 5mM MgCl2, 10% (w/v) polyethylene glycol, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
