3AQ5
Crystal structure of truncated hemoglobin from Tetrahymena pyriformis, Fe(II)-O2 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 69.355, 69.355, 353.761 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.270 - 1.780 |
| R-factor | 0.18775 |
| Rwork | 0.187 |
| R-free | 0.20927 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dlw |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.528 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.270 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 48931 | |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 98.3 | 92.7 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.0M ammonium citrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
