3AQ4

Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-01
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 21 21 2
Unit cell lengths	88.710, 89.977, 46.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.800 - 1.800
R-factor	0.20076
Rwork	0.199
R-free	0.24148
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.496
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.800	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	33083
Completeness [%]	99.7	99.57

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	PEG6000, magnesium chloride, HEPES, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K