3AQ1

Open state monomer of a group II chaperonin from methanococcoides burtonii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-12-21
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	63.564, 115.256, 193.864
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.505 - 2.746
R-factor	0.1963
Rwork	0.190
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a6d
RMSD bond length	0.008
RMSD bond angle	1.105
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_222))

Data quality characteristics

	Overall
Low resolution limit [Å]	21.505
High resolution limit [Å]	2.730
Rmerge	0.104
Number of reflections	18736
<I/σ(I)>	19
Completeness [%]	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	1.6M AMSO4, 2.5%(v/v) dioxane, 0.1M Mes/NaOh pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K