3APS
Crystal structure of Trx4 domain of ERdj5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-08 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.060, 33.419, 68.343 |
| Unit cell angles | 90.00, 92.85, 90.00 |
Refinement procedure
| Resolution | 30.020 - 1.900 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24200 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.562 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX (C/D/E) |
| Refinement software | REFMAC (5.5.0085) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.120 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.049 | 0.364 |
| Number of reflections | 20060 | |
| <I/σ(I)> | 16.4 | 3.7 |
| Completeness [%] | 98.6 | 94.7 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 17.5 % PEG 3350, 140MM LISO4, 70MM HEPES PH7.5, 70MM GLYCINE, 12% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
