3APQ
Crystal structure of J-Trx1 fragment of ERdj5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-17 |
| Detector | DIP6040 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.008, 61.179, 62.912 |
| Unit cell angles | 90.00, 106.34, 90.00 |
Refinement procedure
| Resolution | 39.560 - 1.840 |
| R-factor | 0.173 |
| Rwork | 0.170 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.704 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX (C/D/E) |
| Refinement software | REFMAC (5.5.0085) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.560 | 1.940 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.064 | 0.497 |
| Number of reflections | 33995 | |
| <I/σ(I)> | 15.3 | 3.3 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 13% PEG 4000, 6.5% ISOPROPANOL, 65MM HEPES PH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
