3APO
Crystal structure of full-length ERdj5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-06 |
| Detector | DIP6040 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.100, 53.400, 92.300 |
| Unit cell angles | 90.00, 113.40, 90.00 |
Refinement procedure
| Resolution | 45.830 - 2.400 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.323 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX (C/D/E) |
| Refinement software | REFMAC (5.5.0085) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.830 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.098 | 0.536 |
| Number of reflections | 32121 | |
| <I/σ(I)> | 11.5 | 3 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% PEG 8000, 100MM HEPES PH7.5, 8MM CYSTINE, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
