3AP9

Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with Lacto-N-fucopentaose III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2006-02-01
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000000
Spacegroup name	P 21 21 21
Unit cell lengths	46.419, 49.952, 70.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.977 - 1.330
R-factor	0.15773
Rwork	0.157
R-free	0.17824
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ap5
RMSD bond length	0.007
RMSD bond angle	1.242
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.977	1.360
High resolution limit [Å]	1.330	1.330
Rmerge	0.041	0.231
Number of reflections	37685
<I/σ(I)>		9.4
Completeness [%]	98.7	97.4
Redundancy	7	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	0.275mM protein, 10mM Hepes-NaOH, 2.75mM Lacto-N-fucopentaose III, 50mM sodium chloride, 0.5mM DTT, 50mM ammonium fluoride, 12% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K