3AP7

Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain in complex with lactose sialic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2005-11-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000000
Spacegroup name	P 21 21 21
Unit cell lengths	46.360, 49.917, 70.289
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.960 - 1.530
R-factor	0.16493
Rwork	0.164
R-free	0.18828
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ap5
RMSD bond length	0.009
RMSD bond angle	1.299
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.961	1.570
High resolution limit [Å]	1.530	1.530
Rmerge	0.034	0.192
Number of reflections	25280
<I/σ(I)>		12.4
Completeness [%]	99.9	100
Redundancy	12.8	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	0.275mM protein, 10mM Hepes-NaOH, 2.75mM lactose sialic acid, 50mM sodium chloride, 0.5mM DTT, 80mM ammonium fluoride, 7.5% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K