3AP5
Crystal structure of the galectin-8 N-terminal carbohydrate recognition domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-10-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.28000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.055, 76.055, 66.509 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.010 - 1.920 |
| R-factor | 0.20569 |
| Rwork | 0.204 |
| R-free | 0.23651 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3apb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.155 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.021 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.064 | 0.239 |
| Number of reflections | 15467 | |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.5 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.275mM protein, 10mM Hepes-NaOH, 50mM sodium chloride, 0.5mM DTT, 125mM ammonium fluoride, 8% PEG 3350 , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
