3AOT
Crystal structure of juvenile hormone binding protein from silkworm in its apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-02-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 90.898, 90.898, 138.336 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.860 - 2.200 |
| R-factor | 0.23515 |
| Rwork | 0.234 |
| R-free | 0.25453 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aos |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.579 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.059 | 0.336 |
| Number of reflections | 17925 | |
| <I/σ(I)> | 57.3 | 11.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 20.5 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 50% PEG 200, 0.1 M Tris, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
