3AON
Crystal structure of the central axis (NtpD-NtpG) in the catalytic portion of Enterococcus hirae V-type sodium ATPase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9791, 0.9794, 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.787, 68.433, 51.153 |
| Unit cell angles | 90.00, 114.99, 90.00 |
Refinement procedure
| Resolution | 31.980 - 2.000 |
| R-factor | 0.1974 |
| Rwork | 0.194 |
| R-free | 0.25161 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.276 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.160 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.561 |
| Number of reflections | 21698 | |
| <I/σ(I)> | 10.7 | 2.8 |
| Completeness [%] | 97.2 | 96.3 |
| Redundancy | 6.1 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 18% PEG 3350, 0.2 M Sodium Nitrate, 0.1M MES-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
