3AOG
Crystal structure of glutamate dehydrogenase (GdhB) from Thermus thermophilus (Glu bound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-03-05 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 99.733, 106.546, 166.810 |
| Unit cell angles | 82.83, 87.18, 70.60 |
Refinement procedure
| Resolution | 49.770 - 2.100 |
| R-factor | 0.22845 |
| Rwork | 0.226 |
| R-free | 0.27663 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.411 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.770 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 373553 | |
| Completeness [%] | 95.8 | 81.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES-NaOH (pH7.0) 0.6M ammonium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
