3ANJ
Crystal structure of unsaturated glucuronyl hydrolase from Streptcoccus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.844, 53.191, 70.076 |
| Unit cell angles | 90.00, 96.62, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.19013 |
| Rwork | 0.189 |
| R-free | 0.21281 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zzr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.957 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.006 | 0.290 |
| Number of reflections | 28120 | |
| Completeness [%] | 99.0 | 91.9 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 30% PEG200, 0.1M HEPES, 1% PEG3000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
