3ANI
Crystal structure of unsaturated glucuronyl hydrolase mutant D175N from Streptcoccus agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.117, 53.372, 70.165 |
| Unit cell angles | 90.00, 96.51, 90.00 |
Refinement procedure
| Resolution | 29.160 - 2.500 |
| R-factor | 0.19039 |
| Rwork | 0.187 |
| R-free | 0.26002 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zzr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.002 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.064 | 0.352 |
| Number of reflections | 13575 | |
| Completeness [%] | 99.0 | 98.4 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 40% ethylene glycol, 5% PEG 3000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
