3AMT
Crystal structure of the TiaS-tRNA(Ile2)-ATP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2009-01-01 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97898 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 131.105, 131.105, 86.624 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.480 - 2.900 |
R-factor | 0.24 |
Rwork | 0.240 |
R-free | 0.28600 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.100 | 0.407 |
Number of reflections | 36616 | |
<I/σ(I)> | 29.3 | 2.7 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 15.4 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.085M Tris-HCl pH8.5, 0.17M sodium acetate, 28% PEG 4000, 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |