3AML
Structure of the Starch Branching Enzyme I (BEI) from Oryza sativa L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.066, 124.850, 67.880 |
| Unit cell angles | 90.00, 97.29, 90.00 |
Refinement procedure
| Resolution | 21.120 - 1.700 |
| R-factor | 0.14892 |
| Rwork | 0.146 |
| R-free | 0.19592 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m7x |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.246 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.085 | 0.813 |
| Number of reflections | 78213 | |
| <I/σ(I)> | 20.7 | 3.7 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.4 | pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.4K |
