3AMK
Structure of the Starch Branching Enzyme I (BEI) from Oryza sativa L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.894, 114.854, 66.819 |
| Unit cell angles | 90.00, 93.98, 90.00 |
Refinement procedure
| Resolution | 28.830 - 1.900 |
| R-factor | 0.18263 |
| Rwork | 0.181 |
| R-free | 0.22056 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.965 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.089 | 0.611 |
| Number of reflections | 51972 | |
| <I/σ(I)> | 13 | 1.9 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.7 | pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.7K |
