3AM6
Crystal structure of the proton pumping rhodopsin AR2 from marine alga Acetabularia acetabulum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2009-10-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.120, 110.487, 129.116 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.200 - 3.200 |
| R-factor | 0.29 |
| Rwork | 0.290 |
| R-free | 0.32400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.192 | 3.192 |
| Number of reflections | 18284 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 89.5 | 72.2 |
| Redundancy | 4.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | lipid mesophase | 7.5 | 293 | 0.1M Tris-HCl (pH 7.5), 6% 2-methyl-2,4-pentanediol, 14% polyethylene glycol 400, lipid mesophase, temperature 293K |
