3ALZ
Crystal structure of the measles virus hemagglutinin bound to its cellular receptor SLAM (Form I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 208.119, 208.119, 182.127 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.039 - 4.515 |
| R-factor | 0.3263 |
| Rwork | 0.326 |
| R-free | 0.33760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zb6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.566 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.040 | 4.660 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.078 | 0.592 |
| Number of reflections | 14397 | |
| <I/σ(I)> | 15.4 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.5 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES pH6.5, 0.75M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
