3ALP

Cell adhesion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-09
Detector	Bruker DIP-6040
Wavelength(s)	0.9
Spacegroup name	P 21 3
Unit cell lengths	164.924, 164.924, 164.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.989 - 2.804
R-factor	0.1917
Rwork	0.190
R-free	0.22550
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.247
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL2Map
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.068	0.358
Number of reflections	36109
<I/σ(I)>	19.4	4.3
Completeness [%]	97.9	96.9
Redundancy	3.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	296	1% PEG 3350, 50mM Bis-Tris propane, 50mM citric acid, 6% hexane-1,6-diol , VAPOR DIFFUSION, HANGING DROP, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	5	296	1% PEG 3350, 50mM Bis-Tris propane, 50mM citric acid, 6% hexane-1,6-diol , VAPOR DIFFUSION, HANGING DROP, temperature 296K