3AL2
Crystal Structure of TopBP1 BRCT7/8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-11 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.583, 32.748, 81.762 |
Unit cell angles | 90.00, 109.32, 90.00 |
Refinement procedure
Resolution | 31.020 - 2.000 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.20400 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.346 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.081 | 0.032 | 0.332 |
Number of reflections | 33711 | ||
<I/σ(I)> | 14.068 | ||
Completeness [%] | 99.1 | 99.8 | 92.9 |
Redundancy | 3.6 | 4 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | Li2SO4, Tris-HCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |