3AKT
Crystal structure of xylanase from Trichoderma longibrachiatum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-02 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.8000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.765, 38.841, 81.337 |
| Unit cell angles | 90.00, 94.78, 90.00 |
Refinement procedure
| Resolution | 21.630 - 1.000 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3akq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.082 | 0.490 |
| Number of reflections | 187076 | |
| <I/σ(I)> | 7.7 | 3.3 |
| Completeness [%] | 98.1 | 96.1 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | oil microbatch | 8.5 | 293 | 30% PEG 4000, 0.2M sodium acetate, pH 8.5, oil microbatch, temperature 293K |
