3AKL
Crystal structure of A Helicobacter pylori proinflammatory kinase CtkA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.133, 74.835, 75.815 |
| Unit cell angles | 78.84, 71.36, 85.44 |
Refinement procedure
| Resolution | 19.936 - 2.900 |
| R-factor | 0.1938 |
| Rwork | 0.189 |
| R-free | 0.27960 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.139 | 0.360 |
| Number of reflections | 27093 | |
| <I/σ(I)> | 19.816 | |
| Completeness [%] | 98.4 | 94.1 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 295 | 100 mM sodium acetate, 0.2M sodium thiocyanate, 20% (v/v) PEG 3350, pH 5.5, VAPOR DIFFUSION, temperature 295K |
| 1 | VAPOR DIFFUSION | 5.5 | 295 | 100 mM sodium acetate, 0.2M sodium thiocyanate, 20% (v/v) PEG 3350, pH 5.5, VAPOR DIFFUSION, temperature 295K |
