3AKG
Crystal structure of exo-1,5-alpha-L-arabinofuranosidase complexed with alpha-1,5-L-arabinofuranobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-06-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.052, 91.765, 135.053 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.420 - 1.800 |
R-factor | 0.18876 |
Rwork | 0.187 |
R-free | 0.22053 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3akf |
RMSD bond length | 0.011 |
RMSD bond angle | 1.256 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.420 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.057 | 0.267 |
Number of reflections | 47867 | |
<I/σ(I)> | 52.7 | 9.6 |
Completeness [%] | 99.3 | 98.8 |
Redundancy | 13.9 | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.8M sodium citrate, 0.2M sodium chloride, 0.1M Tris, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |