3AKF
Crystal structure of exo-1,5-alpha-L-arabinofuranosidase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-05-23 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.978, 0.97934, 0.97966, 0.98400, 0.96400 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.961, 91.481, 135.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.810 - 2.200 |
| R-factor | 0.21002 |
| Rwork | 0.208 |
| R-free | 0.25360 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.810 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.081 | 0.285 |
| Number of reflections | 26313 | |
| <I/σ(I)> | 44.4 | 3.1 |
| Completeness [%] | 97.5 | 97.5 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.8M sodium citrate, 0.2M sodium chloride, 0.1M Tris, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
