3AKF

Crystal structure of exo-1,5-alpha-L-arabinofuranosidase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	95
Detector technology	CCD
Collection date	2007-05-23
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.978, 0.97934, 0.97966, 0.98400, 0.96400
Spacegroup name	P 21 21 21
Unit cell lengths	40.961, 91.481, 135.451
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.810 - 2.200
R-factor	0.21002
Rwork	0.208
R-free	0.25360
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.390
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.810	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.081	0.285
Number of reflections	26313
<I/σ(I)>	44.4	3.1
Completeness [%]	97.5	97.5
Redundancy	7	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.8M sodium citrate, 0.2M sodium chloride, 0.1M Tris, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K