3AJY
Crystal Structure of Ancestral Congerin Con-anc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-07 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.777, 53.885, 132.985 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.850 - 2.010 |
| R-factor | 0.20801 |
| Rwork | 0.206 |
| R-free | 0.24687 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c1f |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.435 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 20146 | |
| <I/σ(I)> | 42.8 | 6.7 |
| Completeness [%] | 98.2 | 89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.51 | 293 | 0. 1M MMT, 25%(w/v) PEG1500, 1mM lactose, pH 6.51, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
