3AJO
Crystal structure of wild-type human ferritin H chain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-02-22 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.723, 182.723, 182.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.937 - 1.520 |
| R-factor | 0.1714 |
| Rwork | 0.171 |
| R-free | 0.18320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fha |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.978 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.049 | 0.352 |
| Number of reflections | 40578 | |
| <I/σ(I)> | 9.6 | 2.71 |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 30.6 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 2.0M magnesium chloride, 0.1M Bicine (pH 9.0), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
