3AJL
Crystal structure of d(CGCGGATf5UCGCG): 5-Formyluridine:guanosine Base-pair in B-DNA with DAPI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.003, 40.501, 66.773 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.860 - 2.700 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 355d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | DPS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.700 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.069 | 0.378 |
| Number of reflections | 2342 | |
| <I/σ(I)> | 6.7 | 2.2 |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20mM Na cacodylate, 6mM spermine, 40mM NaCl, 10mM MgCl2, 5% MPD, 0.5mM DAPI, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
