3AJ4
Crystal structure of the PH domain of Evectin-2 from human complexed with O-phospho-L-serine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.747, 48.449, 64.323 |
| Unit cell angles | 90.00, 92.20, 90.00 |
Refinement procedure
| Resolution | 14.450 - 1.000 |
| R-factor | 0.10606 |
| Rwork | 0.105 |
| R-free | 0.12563 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dhi |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.944 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.054 | 0.290 |
| Number of reflections | 102125 | |
| <I/σ(I)> | 46.8 | 8.5 |
| Completeness [%] | 97.1 | 95.3 |
| Redundancy | 6.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |
