3AIX
Crystal structure of PCNA2-PCNA3 complex from Sulfolobus tokodaii (I222)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 91.100, 111.800, 170.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.301 - 2.900 |
| R-factor | 0.2123 |
| Rwork | 0.210 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2izo |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.470 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.549 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 19736 | |
| <I/σ(I)> | 19.7 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.6M ammonium sulfate, 0.1M sodium Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
