3AI7
Crystal Structure of Bifidobacterium Longum Phosphoketolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 142.260, 184.410, 157.440 |
| Unit cell angles | 90.00, 97.31, 90.00 |
Refinement procedure
| Resolution | 47.870 - 2.200 |
| R-factor | 0.14981 |
| Rwork | 0.147 |
| R-free | 0.20552 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | the roughly refined crystal structure of the selenomethionine substituted protein |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.933 |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 156.160 | 2.300 | |
| High resolution limit [Å] | 2.200 | 10.000 | 2.200 |
| Rmerge | 0.085 | 0.037 | 0.290 |
| Number of reflections | 401973 | 4378 | 47288 |
| <I/σ(I)> | 28.5 | 4.6 | |
| Completeness [%] | 98.9 | 98.1 | 93.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 6.6 | 299 | PEG3350, MES, Sodium thiocyanate, pH 6.6, hanging drop, temperature 299K |
