3AHZ
Crystal structure of beta-glucosidase from termite Neotermes koshunensis in complex with Tris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.959, 68.451, 75.228 |
| Unit cell angles | 90.00, 95.53, 90.00 |
Refinement procedure
| Resolution | 25.600 - 1.340 |
| R-factor | 0.11671 |
| Rwork | 0.115 |
| R-free | 0.14381 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cbg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.525 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.390 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.054 | 0.469 |
| Number of reflections | 105222 | |
| <I/σ(I)> | 29.7 | 3.9 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 5.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Bis-Tris, 18-21%(w/v) PEG 3350, 0.1-0.25M MgCl2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
