3AHM
Pz peptidase a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2006-11-01 |
Detector | RIGAKU RAXIS VII |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.634, 193.841, 60.242 |
Unit cell angles | 90.00, 106.54, 90.00 |
Refinement procedure
Resolution | 29.170 - 2.000 |
R-factor | 0.19293 |
Rwork | 0.192 |
R-free | 0.20431 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.166 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.170 |
High resolution limit [Å] | 2.000 |
Number of reflections | 78239 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | The purified protein solution, concentrated to about 20 mg/ml in 50 mM Tris-HCl (pH7.5), was incubated in 12% (w/v) PEG 4000, 0.5 M magnesium acetate, and 0.1 M Tris-HCl (pH 7.0) for five days by the hanging-drop vapor diffusion method at 293 K, VAPOR DIFFUSION, HANGING DROP |