3AHM
Pz peptidase a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-01 |
| Detector | RIGAKU RAXIS VII |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.634, 193.841, 60.242 |
| Unit cell angles | 90.00, 106.54, 90.00 |
Refinement procedure
| Resolution | 29.170 - 2.000 |
| R-factor | 0.19293 |
| Rwork | 0.192 |
| R-free | 0.20431 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.166 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.170 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 78239 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | The purified protein solution, concentrated to about 20 mg/ml in 50 mM Tris-HCl (pH7.5), was incubated in 12% (w/v) PEG 4000, 0.5 M magnesium acetate, and 0.1 M Tris-HCl (pH 7.0) for five days by the hanging-drop vapor diffusion method at 293 K, VAPOR DIFFUSION, HANGING DROP |
