3AHA
Crystal structure of the complex between gp41 fragments N36 and C34 mutant N126K/E137Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.632, 50.479, 56.154 |
| Unit cell angles | 90.00, 90.88, 90.00 |
Refinement procedure
| Resolution | 23.880 - 1.700 |
| R-factor | 0.19021 |
| Rwork | 0.189 |
| R-free | 0.21933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aik |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.989 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.072 | |
| Number of reflections | 27434 | |
| Completeness [%] | 99.1 | 99.95 |
| Redundancy | 3.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 80mM ammonium chloride, 16% 2-methylpentan-2,4-diol, 25% isopropanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
