3AGV
Crystal structure of a human IgG-aptamer complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.587, 107.238, 79.458 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.370 - 2.150 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fc1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.081 | 0.433 |
| Number of reflections | 38726 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 98.3 | 89.1 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | The solution stirring technique with a rotary shaker produced the best diffracting crystals, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
