3AGP

Structure of viral polymerase form I

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2008-11-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9790, 0.9793, 0.9641
Spacegroup name	C 2 2 21
Unit cell lengths	139.498, 256.428, 100.961
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 - 2.800
Rwork	0.215
R-free	0.28300
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	0.917
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.087	0.325
Number of reflections	44610
Completeness [%]	98.2	95.8
Redundancy	6.8	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	30% PEG400, 0.2M calcium acetate, 0.1M HEPES, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
2